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详细介绍
Phagedisplay system pComb3XTT
Cat No. PDS27-9401-05
Introduction
pComb3X is the newest of the pComb vectors. Improvements over pComb3 include increased stability and introduction of SfiI cassette for cloning of full Fab, scFv, peptide and other protein for phage display. 6xHis and HA tags allow for purification and detection. An amber stop codon was introduced to turn-off expression of the pIII fusion protein by switching to a non-supressor strain of E. coli allowing production of soluble protein without subcloning. Alternatively, the gene for phage protein pIII can be removed by SpeI/NheI digest. pComb3XSS is the vector we normally ship. The “TT” refers to the human Fab to tetanus toxin from which the kappa light chain constant region and Fd can be amplified.
【系统内容】
A．表达宿主菌E.coli XL1-Blue（15％甘油保存），800ul/支；
B．噬菌粒载体pComb3 XTT（DNA溶于TE Buffer，浓度50ng/ul），30ul/支；
C．辅助噬菌体VCSM13（滴度为1.2×1013pfu/ml），800ul/支
D. 《运用pcomb3XTT展示系统建库适用操作手册》
【保存温度】
噬菌粒载体、宿主菌保存于-20℃。
辅助噬菌体请存放在4℃即可，不可冻存。
